|A mutation in subunit I of cytochrome oxidase from Rhodobacter sphaeroides results in an increase in steady-state activity but completely eliminates proton pumping|
AS Pawate, J Morgan, A Namslauer, D Mills, P Brzezinski, ...
Biochemistry 41 (45), 13417-13423, 2002
|Redox-coupled proton translocation in biological systems: Proton shuttling in cytochrome c oxidase|
A Namslauer, AS Pawate, RB Gennis, P Brzezinski
Proceedings of the National Academy of Sciences of the United States of …, 2003
|Transmembrane charge separation during the ferryl-oxo→ oxidized transition in a nonpumping mutant of cytochrome c oxidase|
SA Siletsky, AS Pawate, K Weiss, RB Gennis, AA Konstantinov
Journal of Biological Chemistry 279 (50), 52558-52565, 2004
|Controlled uncoupling and recoupling of proton pumping in cytochrome c oxidase|
G Brändén, AS Pawate, RB Gennis, P Brzezinski
Proceedings of the National Academy of Sciences of the United States of …, 2006
|Fabrication of X-ray compatible microfluidic platforms for protein crystallization|
S Guha, SL Perry, AS Pawate, PJA Kenis
Sensors and Actuators B: Chemical 174, 1-9, 2012
|Replacing Asn207 by aspartate at the neck of the D channel in the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides results in decoupling the proton pump|
D Han, A Namslauer, A Pawate, JE Morgan, S Nagy, AS Vakkasoglu, ...
Biochemistry 45 (47), 14064-14074, 2006
|A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction|
SL Perry, S Guha, AS Pawate, A Bhaskarla, V Agarwal, SK Nair, ...
Lab on a Chip 13 (16), 3183-3187, 2013
|Chemical analysis of drug biocrystals: a role for counterion transport pathways in intracellular drug disposition|
RK Keswani, J Baik, L Yeomans, C Hitzman, AM Johnson, AS Pawate, ...
Molecular pharmaceutics 12 (7), 2528-2536, 2015
|X-ray transparent microfluidic chip for mesophase-based crystallization of membrane proteins and on-chip structure determination|
DS Khvostichenko, JM Schieferstein, AS Pawate, PD Laible, PJA Kenis
Crystal growth & design 14 (10), 4886-4890, 2014
|Decoupling Mutations in the D-Channel of the aa3-Type Cytochrome c Oxidase from Rhodobacter sphaeroides Suggest That a Continuous Hydrogen-Bonded …|
J Zhu, H Han, A Pawate, RB Gennis
Biochemistry 49 (21), 4476-4482, 2010
|Mutations which decouple the proton pump of the cytochrome< i> c</i> oxidase from< i> Rhodobacter sphaeroides</i> perturb the environment of glutamate 286|
AS Vakkasoglu, JE Morgan, D Han, AS Pawate, RB Gennis
FEBS letters 580 (19), 4613-4617, 2006
|In situ serial Laue diffraction on a microfluidic crystallization device|
SL Perry, S Guha, AS Pawate, R Henning, I Kosheleva, V Srajer, ...
Journal of applied crystallography 47 (6), 1975-1982, 2014
|An X-ray transparent microfluidic platform for screening of the phase behavior of lipidic mesophases|
DS Khvostichenko, E Kondrashkina, SL Perry, AS Pawate, K Brister, ...
Analyst 138 (18), 5384-5395, 2013
|Towards time-resolved serial crystallography in a microfluidic device|
AS Pawate, V Šrajer, J Schieferstein, S Guha, R Henning, I Kosheleva, ...
Acta Crystallographica Section F: Structural Biology Communications 71 (7 …, 2015
|Crystallization optimization of pharmaceutical solid forms with X-ray compatible microfluidic platforms|
EM Horstman, S Goyal, A Pawate, G Lee, GGZ Zhang, Y Gong, PJA Kenis
Crystal Growth & Design 15 (3), 1201-1209, 2015
|Flash-photolysis of fully reduced and mixed-valence CO-bound Rhodobacter sphaeroides cytochrome c oxidase: heme spectral shifts|
I Szundi, J Ray, A Pawate, RB Gennis, Ó Einarsdóttir
Biochemistry 46 (44), 12568-12578, 2007
|A method of cryoprotection for protein crystallography by using a microfluidic chip and its application for in situ X-ray diffraction measurements|
M Maeki, AS Pawate, K Yamashita, M Kawamoto, M Tokeshi, PJA Kenis, ...
Analytical chemistry 87 (8), 4194-4200, 2015
|A microfluidic-based protein crystallization method in 10 micrometer-sized crystallization space|
M Maeki, S Yamazaki, AS Pawate, A Ishida, H Tani, K Yamashita, ...
CrystEngComm 18 (40), 7722-7727, 2016
|X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds|
JM Schieferstein, AS Pawate, MJ Varel, S Guha, I Astrauskaite, ...
Lab on a Chip 18 (6), 944-954, 2018
|X-ray transparent microfluidic chips for high-throughput screening and optimization of in meso membrane protein crystallization|
JM Schieferstein, AS Pawate, C Sun, F Wan, PN Sheraden, J Broecker, ...
Biomicrofluidics 11 (2), 024118, 2017